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To Pick a Peck of Orange Peppers

From Molecular Variation to Macroscopic Change

By Pamela Kalas, Fatima Syed

To Pick a Peck of Orange Peppers


 

Abstract

This illustrated, interrupted case study explores the genetic and molecular mechanisms leading to color variation in bell peppers. Students are introduced to the roles of several genes involved in the fruit maturation process, with a focus on the biochemical pathway responsible for producing carotenoid pigments. They then make predictions about phenotypic outcomes of particular mutations, propose hypotheses about the molecular basis for orange fruit color, and analyze data from a primary article to evaluate the strength of their hypotheses. Students extend their understanding of what a phenotype is and how dominance relationships between alleles can be rigorously defined. They also apply their knowledge of fundamental processes (e.g., transcription, translation, protein function) to both hypothetical and real-life examples and confront unexpected data that sometimes raise more questions than they answer. The case is presented as a PowerPoint slide deck with a set of student handouts. It also includes a pre-class preparatory reading with an associated quiz, and a set of sample assessment questions. Originally developed for a first-year biology class for majors, the case is also suitable for introductory genetics or molecular genetics courses.

   

Date Posted

10/24/2022

Overview

Objectives

  • Describe how variation in macroscopic and morphological phenotypes can result from variation at the molecular or biochemical levels.
  • Systematically define the dominance relationship between pairs of alleles of a given gene with respect to a phenotype.
  • Use a biochemical pathway chart to predict the potential phenotypic outcomes of mutations at loci encoding particular pathway proteins and, conversely, propose hypotheses about the molecular and biochemical cause(s) of given phenotypes.
  • Describe, analyze, and interpret data (e.g., northern blots, RNA and pigment quantifications, DNA sequencing) from primary literature, evaluate whether they support a given hypothesis, and (if necessary) propose a revised hypothesis.

Keywords

Biosynthesis pathway; data analysis; mutations; pepper color variation; phenotype; dominance; biochemical pathway; carotenoid; pigment; pepper

  

Subject Headings

Genetics / Heredity
Molecular Biology

EDUCATIONAL LEVEL

Undergraduate lower division, Undergraduate upper division

  

FORMAT

PPTX, PDF

   

TOPICAL AREAS

N/A

   

LANGUAGE

English

   

TYPE/METHODS

Journal, Interrupted

 

 

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