This integrative, cross-curricular lab engages middle school biology students in an exercise involving ecology, arthropod biology, and mathematics. Students research the anatomy and behavioral patterns of a species of brine shrimp, compare the anatomy of adult and juvenile brine shrimp, and graph and interpret results. In this article, the authors also provide differentiated learning and enrichment activities for advanced mathematics classes that introduce correlation analysis, statistics, and the importance of automated data collection and sample replicates.