The purpose of this article is to describe a lesson aimed at teaching students the function of enzymes in order to obtain mastery of NGSS Standard HS-LS1-2. The lesson is designed to engage learners through the frameworks of the 5E process and sensemaking. To this end, we will describe the lesson features including an “engage” activity, scientific testing, and technology integration activities including a stop motion video simulation. Furthermore, this lesson allows all students to obtain and demonstrate mastery of enzymatic chemical reactions through the intentional pedagogical strategies of multiple representations and expressions.